Chikungunya IgG/IgM Rapid Test Kit (Colloidal Gold)

I-Chikungunya lusulelo olunqabileyo lwentsholongwane olusasazwa ngokulunywa yingcongconi i-Aedes aegypti eyosulelekileyo.Ibonakala ngerhashalala, umkhuhlane, kunye neentlungu ezibuhlungu zamalungu (arthralgias) ezidla ngokuthatha iintsuku ezintathu ukuya kwezisixhenxe.Eli gama lithatyathwe kwigama lesiMakonde elithetha “into egobileyo” xa kubhekiswa kwindlela yokuma egobileyo eyenziwa ngenxa yeempawu zesi sifo.Kwenzeka ngexesha lemvula kwiindawo ezishushu zehlabathi, ikakhulu e-Afrika, eMzantsi-Mpuma Asia, emazantsi eIndiya nasePakistan.Iimpawu zihlala zingacaciswanga ngokwezonyango ezo zijongwe kumkhuhlane wedengue.Ngokwenene, usulelo oluphindwe kabini lwedengue kunye ne-chikungunya kuye kwaxelwa eIndiya.Ngokungafaniyo nedengue, iimpawu zokopha azifane zifane kwaye amaxesha amaninzi esi sifo sisigulo esizithintelayo.Ngoko ke kubaluleke kakhulu ukwahlula ngokwezonyango idengue kusulelo lwe-CHIK.I-CHIK ifunyanwe ngokusekelwe kuhlalutyo lwe-serological kunye nokuhlukaniswa kwentsholongwane kwiigundane okanye kwinkcubeko ye-tissue.I-IgM immunoassay yeyona ndlela isebenzayo yokuvavanya elebhu.I-Chikungunya IgG / IgM Rapid Test isebenzisa i-antigens edibeneyo ephuma kwiprotheni yesakhiwo sayo, ifumanisa i-IgG / IgM i-anti-CHIK kwi-serum yesigulane okanye kwi-plasma kwimizuzu engama-20.Uvavanyo lunokwenziwa ngabangaqeqeshwanga okanye

abasebenzi abanezakhono ezingephi, ngaphandle kwezixhobo zaselabhoratri ezinzima.

IChikungunya IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equlethe iChikungunya recombinant envelope antigens ehlanganiswe negolide ye-colloid (i-dengue conjugates) kunye nomvundla we-IgG-gold conjugates, 2) i-nitrocellulose inwebu ye-membrane equlethe iibhendi ezimbini zovavanyo (i-G kunye ne-M band band) kunye nolawulo lwe-band).I-G band ifakwe ngaphambili kunye ne-antibody ukuze kubonwe intsholongwane ye-IgG ye-anti-Chikungunya, i-M band ifakwe i-antibody ukuze ibonwe i-IgM ye-anti-Chikungunya virus, kwaye i-C band ifakwe ngaphambili ngebhokhwe yokulwa nomvundla i-IgG.

Xa umthamo owaneleyo wovavanyo lovavanyo lukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.Intsholongwane ye-IgG ye-anti-Chikungunya ukuba ikhona kumzekelo iya kubophelela kwi-Chikungunya conjugates.I-reagents yamanani eebhetshi ahlukeneyo ayinakusetyenziswa ngokutshintshanayo.I-immunocomplex ibanjwe yi-reagent efakwe kwi-G band, eyenza i-burgundy enemibala ye-G band, ebonisa i-Chikungunya virus ye-IgG isiphumo sovavanyo oluhle kwaye iphakamisa usulelo lwamva nje okanye oluphindayo.Intsholongwane ye-IgM ye-anti-Chikungunya, ukuba ikhona kumzekelo, iya kubophelela kwi-Chikungunya conjugates.I-immunocomplex iye ibanjwe yi-reagent efakwe ngaphambili kwi-M band, yenza i-burgundy enemibala ye-M band, ebonisa i-Chikungunya virus ye-IgM isiphumo sovavanyo olulungileyo kwaye iphakamisa usulelo olutsha.Ukungabikho naziphi na iibhendi zovavanyo (G kunye no-M) icebisa isiphumo esibi. Uvavanyo luqulathe ulawulo lwangaphakathi (C band) ekufuneka ibonise band burgundy enombala we immunocomplex yebhokhwe anti umvundla IgG/umvundla IgG-gold conjugate kungakhathaliseki ukuba uphuhliso umbala kuyo nayiphi na bands T.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.