Chlamydia Pneumoniae IgG/IgM Rapid Test Kit (Colloidal Gold)

I-Chlamydia pneumoniae (C. pneumoniae) luhlobo oluqhelekileyo lwebhaktheriya kunye nonobangela omkhulu wenyumoniya kwihlabathi jikelele.Phantse i-50% yabantu abadala banobungqina bosulelo oludlulileyo ngeminyaka engama-20, kwaye ukuphinda usuleleke kamva ebomini kuyinto eqhelekileyo.Uphononongo oluninzi lucebise unxulumano oluthe ngqo phakathi kosulelo lwe-C. pneumoniae kunye nezinye izifo ezivuthayo ezifana ne-atherosclerosis, ukuqina okubukhali kwe-COPD, kunye ne-asthma.

Ukuxilongwa kosulelo lwe-C. pneumoniae lucelomngeni ngenxa yendalo ekhawulezayo ye-pathogen, i-seroprevalence enkulu, kunye nokuba nokwenzeka kwe-transient asymptomatic carriage.Iindlela zokuxilonga ezisekiweyo zelebhu ziquka ukwahlukaniswa kwezinto eziphilayo kwinkcubeko yeseli, uvavanyo lwe-serological kunye novavanyo lwe-PCR.Microimmunofluorescence (MIF), ngumgangatho wangoku "umgangatho wegolide" wokuxilongwa kwe-serological, kodwa uvavanyo lusenalo ulungelelwaniso kwaye lunzima kakhulu.I-Antibody immunoassays lolona vavanyo luqhelekileyo lwe-serology olusetyenziswayo kwaye usulelo oluphambili lwe-chlamydial lubonakaliswa yimpendulo ye-IgM ephambili kwiiveki ezi-2 ukuya kwezi-4 kunye nokulibaziseka kwe-IgG kunye ne-IgA impendulo kwiiveki ezi-6 ukuya kwezi-8.Nangona kunjalo, ekosulelweni kwakhona, amanqanaba e-IgG kunye ne-IgA anyuka ngokukhawuleza, rhoqo kwiiveki ezi-1-2 ngelixa amanqanaba e-IgM enokungafane afunyanwe.Ngesi sizathu, izilwa-buhlungu ze-IgA zibonise ukuba luphawu oluthembekileyo lwe-immunological losulelo oluphambili, olungapheliyo kunye nolwenzekayo ingakumbi xa ludityaniswe nokubhaqwa kwe-IgM.

Ikhithi yoVavanyo oluKhawulezayo lwe-Chlamydia IgG/IgM isekelwe kumgaqo wovavanyo lomgangatho we-immunochromatographic ukuze kufunyaniswe i-Chlamydia pneumoniae IgG/IgM yamajoni omzimba kwiserum yomntu, iplasma okanye igazi elipheleleyo. e I-Antigen conjugates), i-2) i-nitrocellulose membrane strip equkethe ibhendi yokuvavanya (i-T band) kunye nebhendi yokulawula (i-C band).Ibhendi ye-T iqatywe ngaphambili ngempuku echasene nomntu we-IgG, kwaye ibhendi ye-C iqatywe nge-anti-mouse ye-IgG yebhokhwe.I-strip B iqulathe: 1) i-burgundy colored conjugate pad equlethe i-C. pneumoniae antigen edityaniswe ne-colloid gold (C. pneumoniae Antigen conjugates), 2) a

i-nitrocellulose membrane strip equlethe ibhendi yovavanyo (i-T band) kunye nebhendi yokulawula (i-C band).Ibhendi ye-T iqatywe ngaphambili ngempuku echasene nomntu we-IgM, kwaye ibhendi ye-C iqatywe nge-anti-mouse ye-IgG yebhokhwe.

Umtya A:Xa umthamo owaneleyo wovavanyo ukhutshelwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo secapillary kwikhasethi.C.I-antibody ye-IgG ukuba ikhona kumzekelo iya kubophelela kwi-C. pneumoniae Antigen conjugates.I-immunocomplex ithi emva koko ibanjwe kwi-membrane yi-antibody ye-IgG ye-Mouse e-anti-human, eyenza ibhendi ye-T enombala we-burgundy,

ebonisa iziphumo zovavanyo lwe-C. pneumoniae IgG.Ukungabikho kwe-T band kubonisa umphumo ongalunganga.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise i-burgundy band enemibala ye-immunocomplex yebhokhwe ye-anti-mouse IgG / i-mouse IgGgold conjugate kungakhathaliseki ukuba kukho i-T band enemibala.Ngaphandle koko, iziphumo zovavanyo

ayisebenzi kwaye umfuziselo kufuneka uphinde uvavanywe ngesinye isixhobo.

I-strip B:Xa umthamo owaneleyo wovavanyo lovavanyo lukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka nge-capillary action kwikhasethi.C.pneumoniae I-antibody ye-IgM ukuba ikhona kumzekelo iya kubophelela kwi-C. pneumoniae Antigen conjugates.I-immunocomplex ithi emva koko ibanjwe kwi-membrane yi-antibody ye-Mouse e-anti-human ye-IgM, eyenza ibhendi ye-T enombala we-burgundy,

ebonisa iziphumo zovavanyo lwe-C. pneumoniae IgM.Ukungabikho kwe-T band kubonisa umphumo ongalunganga.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise i-burgundy band enemibala ye-immunocomplex yebhokhwe ye-anti-mouse IgG / i-mouse IgGgold conjugate kungakhathaliseki ukuba kukho i-T band enemibala.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.