Inkcazo eneenkcukacha
I-Canine parvovirus antigen ikiti yovavanyo olukhawulezayo isebenzisa umgaqo wendlela yesandwich ephindwe kabini ye-antibody ukubona ngokusemgangathweni i-canine parvovirus antigen kwilindle lezinja.Umgangatho wegolide we-dog parvovirus antibody 1 wasetyenziswa njengesiphawuli sesalathisi, kwaye ummandla wokufumanisa (T) kunye nommandla wolawulo (C) kwinwebu ye-nitrocellulose yaqatywa nge-canine parvovirus antibody 2 kunye ne-anti-chicken yeegusha, ngokulandelanayo.Ngexesha lokufumanisa, isampuli i-chromatographic phantsi kweempembelelo ze-capillary.Ukuba isampuli evavanyiweyo iqukethe i-canine parvovirus antigen, i-antibody standard ye-gold 1 yenza i-antigen-antibody complex kunye ne-canine parvovirus, kwaye idibanisa ne-canine parvovirus antibody 2 egxininisekile kwindawo yokufumanisa ngexesha le-chromatography ukwenza "i-antibody 1-antigen-antibody 2" isangweji, okubangelwa indawo ebomvu-ebomvu yokufumanisa (T);Ngokwahlukileyo, akukho bands ezimfusa-bomvu zivela kummandla wokufumanisa (T);Kungakhathaliseki ukuba khona okanye ukungabikho kwe-canine parvovirus antigen kwisampuli, i-IgY complex yenkukhu esemgangathweni yegolide iya kuqhubeka ibekwe phezulu ukuya kwindawo yokulawula (C), kwaye ibhendi ebomvu-purplish iya kuvela.Ibhendi emfusa-bomvu enikezelwe kwindawo yolawulo (C) ngumgangatho wokugweba ukuba inkqubo yechromatography iqhelekile, kwaye ikwasebenza njengomgangatho wolawulo lwangaphakathi kwii-reagents.
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