ISISHWANKATHELO NENKCAZO UVAVANYO
I-Helicobacter pylori inxulumene neentlobo zezifo zesisu ezibandakanya i-dyspepsia engekho isilonda, i-duodenal kunye ne-gastric ulcer kunye ne-gastritis esebenzayo, engapheliyo.Ukuxhaphaka kosulelo lwe-H. pylori kunokudlula i-90% kwizigulane ezineempawu kunye neempawu zezifo zesisu.Uphononongo lwakutsha nje lubonisa unxulumano losulelo lwe-H. pylori nomhlaza wesisu.
I-pylori inokudluliselwa ngokugqithiswa kokutya okanye amanzi angcoliswe yi-fecal matter.Ii-antibiotics ezidibeneyo kunye ne-bismuth compounds zibonakaliswe ukuba zisebenza kakuhle ekunyangeni ukusuleleka kwe-H. Pylori esebenzayo..H.Usulelo lwepylori ngoku lufunyaniswa ngeendlela zovavanyo oluhlaselayo olusekwe kwi-endoscopy kunye ne-biopsy (okt, i-histology, inkcubeko) okanye iindlela zovavanyo ezingangeneleli ezifana novavanyo lwe-urea breath (UBT), uvavanyo lwe-serologic antibody kunye novavanyo lwe-antigen yesitulo.I-UBT ifuna izixhobo zaselebhu ezibizayo kunye nokusetyenziswa kwe-reagent radioactive.Uvavanyo lwe-antibody lwe-Serologic alwahluli phakathi kosulelo olusebenzayo ngoku kunye nokuvezwa kwangaphambili okanye usulelo oluye lwanyangwa.Uvavanyo lwe-antigen yesitulo lubhaqa i-antigen ekhoyo kwindle, ebonisa usulelo olusebenzayo lwe-H. pylori.Ingasetyenziselwa ukubeka esweni ukusebenza konyango kunye nokuphindaphinda kosulelo.I-H. pylori Ag Rapid Test isebenzisa i-colloidal gold conjugated monoclonal anti-H.i-antibody ye-pylori kunye nenye i-anti-H ye-monoclonal.i-antibody ye-pylori ukubona ngokuthe ngqo i-antigen ye-H. pylori ekhoyo kwi-fecal specimen yesigulana esosulelekileyo.Uvavanyo lusebenziseka lula, luchanekile, kwaye umphumo uyafumaneka kwimizuzu eyi-15.
UMGAQO
Uvavanyo lwe-H. pylori Ag Rapid yisandwich lateral flow chromatographic immunoassay.Uluhlu lovavanyo luquka: 1) i-burgundy colored conjugate pad equkethe i-monoclonal anti-H.i-antibody ye-pylori edibeneyo negolide ye-colloidal (i-anti-Hp conjugates) kunye ne-2) i-nitrocellulose membrane strip equkethe umgca wokuvavanya (umgca we-T) kunye nomgca wokulawula (umgca we-C).Umgca we-T uqatywe ngaphambili kunye nenye i-monoclonal anti-H.i-pylori antibody, kunye nomgca we-C uqatywe ngaphambili nge-anti-mouse ye-IgG yebhokhwe.
Xa umthamo owaneleyo we-fecal specimen ekhutshiweyo ikhutshwa kwisampulu yequla lekhasethi yovavanyo, isampuli iyafuduka ngesenzo se-capillary kwikhasethi.I-antigens ye-H. pylori, ukuba ikhona kwi-specimen, iya kubophelela kwi-anti-Hp conjugates.I-immunocomplex ibanjwe kwi-membrane nge-antibody efakwe ngaphambili eyenza umgca we-T burgundy onemibala, ebonisa umphumo we-H. pylori wokuvavanya.Ukungabikho komgca we-T kubonisa ukuba ukuxinwa kwe-antigens ye-H. pylori kwi-specimen ingaphantsi kwinqanaba elibonakalayo, ebonisa isiphumo esibi se-H. pylori. Uvavanyo luqulethe ulawulo lwangaphakathi (umgca we-C) omele ubonise umgca we-burgundy onemibala i-immunocomplex yebhokhwe ye-anti-mouse IgG/mouse IgG-gold conjugate kungakhathaliseki uphuhliso lombala kumgca we-T.Ukuba umgca we-C awuphuhli, umphumo wovavanyo awusebenzi kwaye umboniso kufuneka uphinde uhlolwe ngesinye isixhobo.