Inkcazo eneenkcukacha
Ukuba kukho umlinganiselo othile we-antibody ye-HIV-1 okanye i-antibody ye-HIV-2 kwi-serum, i-antibody ye-HIV kwi-serum kunye ne-antigen ye-gp41 ephinda iphinde iphinde iphinde ibe ne-antigen ye-gp36 ekwileyibhile yegolide iya kufakwa i-immunoconjugated ukwenza ikhompleksi xa ichromatography kwindawo yeleyibhile yegolide.Xa i-chromatography ifikelela kumgca wokuvavanya (umgca we-T1 okanye umgca we-T2), i-complex iya kuba i-immunoconjugated kunye ne-recombinant gp41 antigen efakwe kumgca we-T1 okanye i-recombinant gp36 antigen efakwe kumgca we-T2, ukwenzela ukuba i-bridging yegolide ye-colloidal iya kuba nombala kumgca we-T1 okanye umgca we-T2.Xa iileyibhile zegolide eziseleyo ziqhubeka ne-chromatographed ukuya kumgca wokulawula (umgca we-C), ileyibhile yegolide iya kuba nombala wokusabela komzimba kunye ne-multiantibody efakwe apha, oko kukuthi, zombini umgca we-T kunye nomgca we-C uya kuba nombala njengamabhanti abomvu, ebonisa ukuba i-antibody ye-HIV iqulethwe egazini;Ukuba i-serum ayinayo i-antibody ye-HIV okanye ingaphantsi komlinganiselo othile, i-antigen ye-gp41 okanye i-antigen ye-gp36 kwi-T1 okanye i-T2 ayiyi kusabela, kwaye umgca we-T awuyi kubonisa umbala, ngelixa i-polyclonal antibody kumgca we-C iya kubonisa umbala emva kokusabela komzimba kunye neleyibhile yegolide, ebonisa ukuba akukho antibody ye-HIV egazini.