ISISHWANKATHELO NENKCAZO UVAVANYO
Umkhuhlane sisifo esosulelayo kakhulu, esibukhali, sisifo sentsholongwane sendlela yokuphefumla.Ii-causative agents zesi sifo zi-immunologically ezahlukeneyo, iintsholongwane ze-RNA ezinomgca omnye owaziwa ngokuba ziintsholongwane zomkhuhlane.Zintathu iindidi zeentsholongwane zomkhuhlane: A, B, kunye no-C. Iintsholongwane zohlobo A zezona zixhaphake kakhulu kwaye zinxulunyaniswa nezona zifo ziyingozi kakhulu.Iintsholongwane zoHlobo lwe-B zivelisa isifo esithambileyo ngokubanzi kuneso sibangelwa luhlobo A. Iintsholongwane ze-C azizange zidibaniswe nobhubhani omkhulu wesifo somntu.Zombini iintsholongwane zohlobo A no-B zinokujikeleza ngaxeshanye, kodwa ngokuqhelekileyo uhlobo olunye lulawula ngexesha elithile lonyaka.Ii-antigens zoMkhuhlane zinokufunyanwa kwiisampuli zeklinikhi ngokuhlolwa kwe-immunoassay.Uvavanyo loMkhuhlane A+B luvavanyo lwe-immunoassay olusecaleni olusebenzisa izilwa-buhlungu ze-monoclonal ezinovakalelo ezithe ngqo kwii-antigens zomkhuhlane.Uvavanyo lukhethekileyo kwiintlobo zomkhuhlane A kunye no-B ii-antigens ezingaziwayo ukuba ziphinde zisebenze kwizityalo eziqhelekileyo okanye ezinye iipathogens zokuphefumla.
Intsholongwane yokuphefumla i-Syncytial Virus(RSV) ngoyena nobangela uxhaphakileyo we-bronchiolitis kunye nenyumoniya phakathi kweentsana kunye nabantwana abangaphantsi komnyaka omnye ubudala.I-III iqala rhoqo ngomkhuhlane, impumlo evuzayo, ukukhohlela kwaye ngamanye amaxesha ukutswina.Isifo esiqatha esisezantsi sokuphefumla singenzeka nakweyiphi na iminyaka, ngakumbi kubantu abadala okanye phakathi kwabo banentliziyo ephazamisekileyo, imiphunga okanye amajoni omzimba.RSV isasazeka ukusuka
ukukhutshwa kokuphefumla ngokunxibelelana ngokusondeleyo nabantu abosulelekileyo okanye ukudibana nezinto ezingcolisekileyo okanye izinto.
UMGAQO
I-Influenza A/B+RSV Antigen Rapid Test Kit isekelwe kumgaqo wovavanyo olusemgangathweni lwe-immunochromatographic ekumiselweni kwe-antigens zoMkhuhlane A/B+RSV kumzekelo weNasal Sawb.Ngexesha lovavanyo, isampuli ekhutshiweyo isabela kunye ne-anti-influenza A kunye no-B antibodies ezidityaniswe kumasuntswana anemibala kwaye zifakwe ngaphambili kwisampulu yovavanyo.Umxube ke ufuduka kwi-membrane ngesenzo se-capillary kwaye udibanise ne-reagents kwi-membrane.Ukuba kukho ii-antigens zentsholongwane engu-A kunye ne-B ezaneleyo kulo mzekelo, i(s) ezinemibala ziyakwenzeka kwindawo yovavanyo yenwebu.I-Strip B iqukethe: 1) i-burgundy colored conjugate pad equkethe i-antigen edibeneyo ehlanganiswe negolide ye-colloid (i-monoclonal mouse anti Respiratory Syncytial Virus (RSV) i-antibody conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocelland band band equkethe i-band band ye-nitrocell kunye ne-band yovavanyo T).I-T band ifakwe ngaphambili kunye ne-monoclonal mouse anti-Respiratory Syncytial Virus (RSV) i-antibody ukuze kubonwe i-Respiratory Syncytial Virus (RSV) glycoprotein F antigen, kwaye i-C band ifakwe ngaphambili kunye nebhokhwe yokunqanda umvundla i-IgG.
I-Strip A: Umxube ke ufuduka kwi-membrane ngesenzo se-capillary kwaye udibanise ne-reagents kwi-membrane.Ukuba kukho ii-antigens zentsholongwane engu-A kunye ne-B ezaneleyo kulo mzekelo, i(s) ezinemibala ziyakwenzeka kwindawo yovavanyo yenwebu.Ubukho bebhanti enemibala kwingingqi ye-A kunye / okanye ye-B ibonisa umphumo ochanekileyo we-antigens ethile yentsholongwane, ngelixa ukungabikho kwayo kubonisa umphumo ombi.Ukubonakala kwebhanti enemibala kwindawo yokulawula isebenza njengolawulo lwenkqubo, ebonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.
I-strip B:Xa umthamo owaneleyo wovavanyo ukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka nge-capillary action kwikhasethi.I-Respiratory Syncytial Virus(RSV) ukuba ikhona kwi-specimen iya kubophelela kwi-monoclonal mouse anti-Respiratory Syncytial Virus(RSV) antibody conjugates.I-immunocomplex ke ibanjwe kwi-membrane yi-anti-coated mouse anti-Respiratory Syncytial Virus (RSV) antibody, eyenza i-T band enemibala e-burgundy, ebonisa i-Respiratory Syncytial Virus (RSV) i-antigen positive test result.Ukungabikho kwebhendi yovavanyo (T) icebisa isiphumo esibi.Uvavanyo iqulathe ulawulo lwangaphakathi (C band) ekufuneka abonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG/umvundla IgG-igolide conjugate kungakhathaliseki ukuba uphuhliso umbala kuyo nayiphi na bands uvavanyo.Ngaphandle koko