ISISHWANKATHELO NENKCAZO UVAVANYO
I-Visceral leishmaniasis, okanye i-Kala-azar, lusulelo olusasazwayo olubangelwa zintlobo ezininzi ze-L. donovani.Esi sifo siqikelelwa yiWorld Health Organisation (WHO) ukuba sichaphazela abantu abamalunga nezigidi ezili-12 kumazwe angama-88.Isasazeka ebantwini ngokulunywa ziimpukane zesanti ze-Phlebotomus, ezithi zifumane usulelo ngokutya izilwanyana ezosulelekileyo.Nangona isisifo esifumaneka kumazwe ahlwempuzekileyo, kuMazantsi Yurophu, iye yaba sesona sigulo sixhaphakileyo kubaguli bakaGawulayo.Ukuchongwa kwe-L. donovani organism kwigazi, umongo wethambo, isibindi, i-lymph nodes okanye i-spleen inikeza indlela ecacileyo yokuxilongwa.Ukufunyanwa kwe-serological ye-anti-L.I-donovani IgM ifunyenwe njengeyona nto ibalaseleyo umakishayo kwi-acute Visceral leishmaniasis.Uvavanyo olusetyenziswa eklinikhi lubandakanya i-ELISA, i-antibody ye-fluorescent okanye uvavanyo oluthe ngqo lwe-agglutination 4-5.Kutshanje, ukusetyenziswa kwe-L. donovani iprotheyini ethile kuvavanyo kuye kwaphucula ubuntununtunu kunye neenkcukacha ngokumangalisayo.
I-Leishmania IgG/IgM Combo Rapid Test luvavanyo lwe-protein olusekwe kwi-serological, efumanisa izilwa-buhlungu ze-IgG kunye ne-IgM kwi-L. Donovani ngaxeshanye.Uvavanyo lubonelela ngesiphumo esithembekileyo ngaphakathi kwemizuzu eyi-15 ngaphandle kwezixhobo.
UMGAQO
ILeishmania IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay.Ikhasethi yovavanyo iquka: 1) i-burgundy colored conjugate pad equlethe i-recombinant L. donovani antigen edityaniswe negolide ye-colloid (i-Leishmania conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocellulose strip equlethe iibhendi ezimbini zovavanyo (i-T1 kunye ne-T2 bands) kunye nebhendi yokulawula (i-C band).Ibhendi ye-T1 ifakwe ngaphambili kunye ne-monoclonal anti-human IgM yokukhangela i-anti-L.I-donovani IgM, ibhendi ye-T2 ifakwe ngaphambili kunye ne-reagents yokuchongwa kwe-anti-L.donovani IgG, kunye C band precoated nebhokhwe anti umvundla IgG.
Xa umthamo owaneleyo wesampulu yovavanyo ukhutshwa kwisampulu yequla lekhasethi, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.I-L. donovani IgM ukuba ikhona kumzekelo iya kubophelela kwi-conjugates ye-Leishmania.I-immunocomplex iye ibanjwe kwi-membrane yi-anti-human IgM antibody, eyenza i-burgundy band T1 enemibala, ebonisa i-L. donovani IgM isiphumo sovavanyo oluhle.I-L. donovani IgG ukuba ikhona kumzekelo iya kubophelela kwi-conjugates ye-Leishmania.I-immunocomplex ke ibanjwe yi-reagents efakwe ngaphambili kwi-membrane, yenza i-burgundy enemibala ye-T2 band, ebonisa i-L. donovani IgG isiphumo sovavanyo oluhle.
Ukungabikho kwayo nayiphi na i-T bands (T1 kunye ne-T2) ibonisa umphumo ongalunganga.Uvavanyo iqulathe ulawulo lwangaphakathi (C band) ekufuneka abonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG / umvundla IgG-igolide conjugate kungakhathaliseki uphuhliso lombala kuyo nayiphi na bands T.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
