ISISHWANKATHELO NENKCAZO UVAVANYO
Isifo seengcongconi sisifo seengcongconi, esosulelayo, esosulela abantu abangaphezu kwezigidi ezingama-200 kwaye sibulala abantu abangaphezu kwesigidi esi-1 ngonyaka.Ibangelwa ziindidi ezine zePlasmodium: P. falciparum, P. vivax, P. ovale, kunye neP. malariae.Ezi plasmodia zosulela kwaye zitshabalalise iierythrocyte zabantu, zivelisa iingqele, umkhuhlane, ianemia, kunye nesplenomegaly.I-P. falciparum ibangela isifo esibi kakhulu kunezinye iintlobo ze-plasmodial kwaye ibangela ukufa kwabantu abaninzi kwimalariya, kwaye sesinye sezona zifo zixhaphakileyo zemalariya.
Ngokwemveli, isifo seengcongconi sifunyaniswa ngokuboniswa kwezinto eziphilayo kwi-Giemsa ene-smears eshinyeneyo yegazi le-peripheral, kwaye iintlobo ezahlukeneyo ze-plasmodium zahlulwa ngokubonakala kwazo kwii-erythrocyte ezosulelekileyo.Ubuchwephesha buyakwazi ukuxilonga ngokuchanekileyo nangokuthembekileyo, kodwa kuphela xa kusenziwa zii-microscopists ezinezakhono zisebenzisa iiprothokholi ezichaziweyo, ezibonisa imiqobo emikhulu kwiindawo ezikude nezihluphekayo zehlabathi.
Uvavanyo lwe-Pf Ag Rapid luphuhliselwe ukusombulula le miqobo.Ifumanisa i-Pf ethile ye-antigen pHRP-II kwi-specimen yegazi lomntu.Ingenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwezixhobo zebhubhoratri.
UMGAQO
UVavanyo lwe-Pf Ag oluKhawulezayo luvavanyo lwe-chromatographic olusecaleni.Amacandelo e-strip yokuvavanya aquka: 1) i-burgundy colored conjugate pad equkethe i-antibody ye-monoclonal anti-pHRP-II ehlanganiswe negolide ye-colloid (i-pHRP II-igolide ye-conjugates), i-2) i-nitrocellulose membrane strip equlethe ibhendi yovavanyo (Pf) kunye nebhendi yokulawula (i-C band).Ibhendi ye-Pf ifakwe ngaphambili kunye ne-polyclonal anti-pHRP-II antibodies, kwaye i-C band ifakwe ngaphambili kunye nebhokhwe ye-anti-mouse IgG.
Ngexesha lovavanyo, umthamo owaneleyo wesampuli yegazi unikezelwa kwisampulu kakuhle (S) yekhasethi yovavanyo, i-lysis buffer yongezwa kwi-buffer well (B).Isithinteli sinesicoci esiseza iiseli ezibomvu zegazi kwaye sikhuphe i-antigen ye-PHRPII, ethi ifuduke ngentshukumo ye-capillary ngaphaya komcu ogcinwe kwikhasethi.I-pHRP-II ukuba izipho kumzekelo ziya kubophelela kwi-pHRP II-conjugates yegolide.I-immunocomplex ke ibanjwe kwi-membrane yi-anti-coated polyclonal antipHRP II antibodies, eyenza i-burgundy enemibala ye-Pf band, ebonisa isiphumo sovavanyo oluhle lwe-Pf.
Ukungabikho kwebhendi yePf kuphakamisa isiphumo esibi.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise ibhendi enemibala e-burgundy ye-immunocomplex yebhokhwe anti-mouse IgG / mouse IgG (pHRP II-gold conjugates) kungakhathaliseki uphuhliso lombala kuyo nayiphi na ibhendi ye-Pf.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
