ISISHWANKATHELO NENKCAZO UVAVANYO
I-M.pneumoniae inokubangela intaphane yeempawu ezifana ne-primary atypical pneumonia, tracheobronchitis, kunye nesifo sendlela yokuphefumula ephezulu.I-tracheobronchitis ixhaphake kakhulu kubantwana abanesistim somzimba esincitshisiweyo, kwaye ukuya kuthi ga kwi-18% yabantwana abosulelekileyo bafuna ukulaliswa esibhedlele.Ngokonyango, i-M. pneumoniae ayikwazi ukwahlula kwi-pneumonia ebangelwa ezinye iibhaktheriya okanye iintsholongwane.Ukuxilongwa okucacileyo kubalulekile kuba unyango lwe-M. pneumoniae usulelo kunye ne-antibiotics ye-β-lactam ayisebenzi ngelixa unyango lwe-macrolides okanye i-tetracyclines lunokunciphisa ubude besifo.
Ukubambelela kwi-M. pneumoniae kwi-epithelium yokuphefumula linyathelo lokuqala kwinkqubo yokusuleleka.Le nkqubo yokuncamathela sisiganeko esintsonkothileyo esifuna iiprotheni ezininzi zokuncamathelisa, ezifana ne-P1, i-P30, kunye ne-P116.Isiganeko esiyinyani sosulelo olunxulumene ne-M. pneumoniae alucaci njengoko kunzima ukuxilonga kwizigaba zokuqala zosulelo.
UMGAQO
I-Mycoplasma Pneumoniae IgG/IgM Rapid Test Kit esekwe kumgaqo wovavanyo lomgangatho we-immunochromatographic ukumiselwa kwe-antibody ye-Mycoplasma Pneumoniae IgG/IgM kwiserum yomntu, iplasma okanye igazi elipheleleyo. amasango), i-2) i-nitrocellulose membrane strip equkethe ibhendi yokuvavanya (i-T band) kunye nebhendi yokulawula (i-C band).Ibhendi ye-T ifakwe ngaphambili nge-anti-igG yempuku echasene nomntu, kwaye ibhendi ye-C iqatywe nge-anti-mouse ye-IgG yebhokhwe.I-Strip B iqulethwe: 1) i-burgundy colored conjugate pad equkethe i-antigen ye-MP ehlanganiswe negolide ye-colloid (i-MP Antigen conjugates), i-2) i-membrane ye-nitrocellulose equkethe i-band yokuvavanya (i-T band) kunye nebhendi yokulawula (i-C band).Ibhendi ye-T ifakwe ngaphambili ngempuku echasene nomntu we-IgM, kwaye ibhendi ye-C iqatywe nge-anti-mouse ye-IgG yebhokhwe.
I-Strip A:Xa umthamo owaneleyo wovavanyo wovavanyo unikezelwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary ngapha kwekhasethi.I-antibody ye-MP IgG ukuba ikhona kumzekelo iya kubophelela kwi-MP Antigen conjugates.I-immunocomplex iye ibanjwe kwi-membrane yi-anti-coated Mouse anti-human IgG antibody, eyenza i-T band enemibala e-burgundy, ebonisa isiphumo sovavanyo lwe-MP IgG.Ukungabikho kwe-T band kubonisa umphumo ongalunganga.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise ibhendi enemibala e-burgundy ye-immunocomplex yebhokhwe echasene ne-mouse IgG / i-mouse IgG-gold conjugate kungakhathaliseki ukuba kukho i-T band enemibala.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
I-strip B:Xa umthamo owaneleyo wovavanyo lomzekelo ukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary ngapha kwekhasethi.I-antibody ye-MP IgM ukuba ikhona kumzekelo iya kubophelela kwi-MP Antigen conjugates.I-immunocomplex iye ibanjwe kwi-membrane yi-anti-coated Mouse anti-human IgM antibody, eyenza i-T band enemibala e-burgundy, ebonisa isiphumo sovavanyo lwe-MP IgM.Ukungabikho kwe-T band kubonisa umphumo ongalunganga.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise ibhendi enemibala e-burgundy ye-immunocomplex yebhokhwe echasene ne-mouse IgG / i-mouse IgG-gold conjugate kungakhathaliseki ukuba kukho i-T band enemibala.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.