IDengue fever sisifo esosulelayo esibangelwa yintsholongwane yedengue, esosulela kakhulu ebantwini ngeengcongconi.Ixhaphake kakhulu kwihlabathi jikelele, ibangela izigidi zosulelo kunye namawakawaka okufa minyaka le.Iimpawu zomkhuhlane wedengue ziquka umkhuhlane ophezulu, intloko ebuhlungu, iintlungu zamalungu kunye nezihlunu, kwaye kwiimeko ezinzima, kunokukhokelela ekopheni kunye nokulimala kwamalungu.Ngenxa yosulelo olukhawulezayo noluxhaphakileyo, umkhuhlane wedengue uyingozi enkulu kwimpilo yoluntu nakwintlalontle yehlabathi.
Ukufumanisa ngokukhawuleza nokulawula ukusasazeka kwefiva yedengue, uvavanyo lwentsholongwane olukhawulezileyo noluchanekileyo luye lwabalulekile.Kule nkalo, iikiti zokuxilonga ngokukhawuleza zidlala indima ebalulekileyo.Zisebenziseka lula, izixhobo zovavanyo ezikhawulezayo ezinceda amaziko ezonyango kunye nabaphandi be-epidemiological ekuqinisekiseni ngokukhawuleza ukuba ngaba abantu banentsholongwane ye-dengue.Ngokusebenzisa ezi zixhobo zokuxilonga, oogqirha nabaphandi banokufumanisa kwaye bababeke bodwa abantu abosulelekileyo kwangethuba, baphumeze unyango olufanelekileyo kunye nemilinganiselo yokulawula, ngaloo ndlela bethintela ngokunempumelelo ukusasazeka kwecesina.Ke ngoko, iikiti zokuxilonga ngokukhawuleza zibambe ukubaluleka okubalulekileyo ekuthinteleni nasekulawuleni ukuqhambuka komkhuhlane wedengue.
Umgaqo-siseko wokuSebenza kunye neNkqubo yokuSetyenziswa kweKhithi yokuDinga eKhawulezayo
· Imigaqo esisiseko ye-Antibody-Antigen Reaction
I-antibody-antigen reaction ngumgaqo osisiseko kwi-immunology esetyenziselwa ukuqatshelwa okuthe ngqo kunye nokubopha ii-antigens.Ii-antibodies zibophelela kwii-antigens ukuze zenze i-immune complexes, inkqubo ebophelelayo eqhutywa ngumtsalane kunye nobudlelwane phakathi kwe-antibodies kunye ne-antigens.Kwimeko yekiti yokuhlola i-dengue fever, ii-antibodies zibophelela kwii-antigens ezivela kwi-virus ye-dengue, okubangelwa ukubunjwa kwe-immune complexes ebonakalayo.
· Inkqubo yoVavanyo lweKhithi yoDinga
Inyathelo 1: Yiza nomzekelo kunye nezixhobo zovavanyo kwiqondo lobushushu begumbi ukuba ifriji okanye ikhenkcezisiwe.Emva kokuba inyibilikisiwe, xuba isampuli kakuhle ngaphambi kokuvavanya.
Inyathelo 2: Xa ulungele ukuvavanya, vula isingxobo kwinotshi kwaye ususe isixhobo.Beka isixhobo sovavanyo kwindawo ecocekileyo nesicaba.
Inyathelo lesi-3: Qinisekisa ukuba uleyibhelishe isixhobo ngenombolo yesazisi yomzekelo.
Inyathelo lesi-4: Kuvavanyo lwegazi lonke
- Galela ithontsi legazi eli-1 (malunga ne-30-35 µL) kwisampulu yequla.
- Emva koko yongeza amathontsi ama-2 (malunga ne-60-70 µL) yeSampuli yeDiluent ngoko nangoko.
Kuvavanyo lweserum okanye lweplasma
- Gcwalisa i-pipette dropper kunye nomzekelo.
- Ukubamba idropha ngokuthe nkqo, khupha ithontsi e-1 (malunga ne-30-35 µL) yomzekelo kwisampulu yequla uqinisekisa ukuba akukho maqamza omoya.
-Emva koko yongeza i-2 drops (malunga ne-60-70 µL) yeSampuli yeDiluent ngoko nangoko.
Inyathelo lesi-6: Iziphumo zingafundwa kwimizuzu engama-20.Iziphumo ezincomekayo zinokubonakala ngomzuzu o-1.
Musa ukufunda iziphumo emva kwemizuzu engama-30. Ukuze ugweme ukudideka, lahla isixhobo sokuvavanya emva kokutolika umphumo.
· Ukutolikwa kwesiphumo soVavanyo
1. IZIPHUMO EZINGAMBI: Ukuba kuphela i-C band iphuhlisiwe, uvavanyo lubonisa ukuba inqanaba le-dengue Ag kwi-specimen ayibonakali.Isiphumo sibi okanye singasebenzi.
2. IZIPHUMO EZINESIZO: Ukuba omabini amacandelo C no-T aphuhlisiwe, uvavanyo lubonisa ukuba umfuziselo unedengue Ag.Isiphumo sivumayo okanye siyasebenza.Iisampulu ezineziphumo ezincomekayo kufuneka ziqinisekiswe ngenye indlela (iindlela) zovavanyo ezifana ne-PCR okanye i-ELISA kunye neziphumo zeklinikhi phambi kokuba kwenziwe uqinisekiso oluqinisekileyo.
3. AYISEBENZAYO: Ukuba akukho bhanti ye-C ephuhlisiwe, uvavanyo alusebenzi kungakhathaliseki ukuba uphuhliso lombala lunjani kwibhendi ye-T njengoko kubonisiwe ngezantsi.Phinda uvavanyo ngesixhobo esitsha.
Izinto eziluncedo kwi-BoatBio Dengue Rapid Diagnostic Kit
· Ukukhawuleza
1. Ixesha elicuthiweyo loVavanyo:
Ikhithi yokuxilonga isebenzisa iteknoloji yokuvavanya ngokukhawuleza, ivumela uhlalutyo lwesampulu kunye nokuveliswa kweziphumo ukuba kugqitywe kwimizuzu engama-20.
Xa kuthelekiswa neendlela zaselabhoratri zemveli, ikiti inciphisa kakhulu ixesha lokuvavanya, iphucula ukusebenza kakuhle.
2. Ukufunyanwa kwesiphumo seXesha lokwenyani:
Ikhithi yokuxilonga ibonelela ngeziphumo zexesha lokwenyani kwangoko emva kokwenziwa kwesampula kunye nokugqitywa kokusabela.
Oku kwenza ukuba abaqeqeshi bezonyango benze ngokukhawuleza ukuxilongwa kunye nezigqibo, ukukhawulezisa ukuhlolwa kwezifo kunye neenkqubo zonyango.
· Uvakalelo kunye neNgcaciso
1. Uvakalelo olomeleleyo:
Uyilo lwekhithi luyenza ikwazi ukubona ubukho bentsholongwane yedengue ngovakalelo oluphezulu.
Nakwiisampulu ezinoxinzelelo oluphantsi lwentsholongwane, ikhithi ibona intsholongwane ngokuthembekileyo, iphucula ukuchaneka koxilongo.
2. Ukucacisa okuphezulu:
Izilwa-buhlungu zekhithi zibonisa ukuchaneka okuphezulu, okuzivumela ukuba zibopheleleke ngokukodwa kwintsholongwane yedengue.
Lo mandla wokwahlula wenza ukuba ikhithi ikwazi ukwahlula phakathi kosulelo lwentsholongwane yedengue kunye nezinye iintsholongwane ezinxulumeneyo
(ezifana ne-Zika virus, i-yellow fever virus), ukunciphisa ukuxilongwa kakubi kunye nokungahambi kakuhle.
· Ukusebenziseka ngokulula
1. Amanyathelo alula okusebenza:
Ikiti yokuxilonga ngokuqhelekileyo ibonisa amanyathelo okusebenza ngokuthe ngqo, okwenza abasebenzisi bakwazi ukuziqhelanisa ngokukhawuleza nokusetyenziswa kwayo.
Amanyathelo acacileyo namfutshane abandakanyekayo, kubandakanywa ukudibanisa isampuli, ukuxuba i-reagent, ukuphendula, kunye nesiphumo sokutolika.
2. Akukho mfuneko yesiXhobo esiNgxaki okanye iMiqathango yeLebhu:
Ikhithi yokuxilonga ayifuni izixhobo ezintsonkothileyo okanye iimeko zelebhu ukuze isebenze kunye nokufunda iziphumo.
Oku kuphatheka kunye nokuguquguquka kwenza ikiti ifaneleke kwiimeko ezahlukeneyo, kubandakanywa neendawo ezikude okanye izibonelelo zempilo ezinobuncwane obuncinci.
Isishwankathelo, i-Dengue Rapid Diagnostic Kit inikezela ngeenzuzo ezifana nokukhawuleza, uvakalelo, ukuchaneka, kunye nokulula kokusetyenziswa, okwenza kube sisixhobo esibalulekileyo sokufunyanwa kwentsholongwane ye-dengue echanekileyo kunye nechanekileyo kwiindawo ezahlukeneyo.
Isindululo seMveliso
Ixesha lokuposa: Aug-16-2023