Qrf Antibody Test Iphepha elinganqunyulwanga

Qrf Uvavanyo lwe-Antibody ye-Qrf Iphepha Elinganqunyulwanga Eliphawulweyo

Uvavanyo lwe-Qrf Antibody

Uhlobo: Iphepha elingasikwanga

Uphawu: Bio-mapper

Ikhathalogu: REA0911

Umzekelo: WB/S/P

Ikhithi yokuvavanya intsholongwane yerhashalala yegusha isetyenziselwa ukukhangela izithinteli-gazi zerhashalala kwiserum yeegusha, ezinokuthi zisetyenziswe ukuvavanya amandla omzimba wogonyo lwerhashalala okanye ukuncedisa ekuchongeni isifo.Umgaqo-siseko ngowokuba ikiti yokubona i-antibody virus ye-pox yegusha yenziwe nge-protein ye-protein ye-protein ye-nyukliya egqunywe ngaphambili, iziphawuli ze-enzyme kunye nezinye ii-reagents ezixhasayo, kunye nomgaqo we-enzyme-linked immunoassay (ELISA) isetyenziselwa ukufumanisa i-antibody ye-pox yegusha kwisampulu ye-serum yeegusha.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Inkcazo eneenkcukacha

Ukufunyanwa kwe-antibody yentsholongwane yerhashalala yegusha yenziwe nge-microplate eqatywe ngaphambili ngepox yeprotein ye-nyukliya ye-antigen, iziphawuli ze-enzyme kunye nezinye ii-reagents ezixhasayo, kunye nomgaqo we-enzyme-linked immunoassay (ELISA) isetyenziselwa ukufumanisa i-antibody ye-pox yegusha kwisampulu ye-serum yegusha.Ngexesha lovavanyo, i-serum yokulawula kunye nesampuli yokuhlolwa yongezwa kwipleyiti ye-microplate, kwaye ukuba isampuli iqulethe i-antibody ye-pox yegusha emva kokufukanyelwa, iya kubotshwa kwi-antigen kwi-plate ye-microplate, kunye namanye amacandelo angabotshwanga aya kususwa emva kokuhlamba;Emva koko yongeza i-enzyme marker ukuba ibophe ngokuthe ngqo kwi-antigen-antibody complex kwi-microplate plate;Abamakishi be-enzyme abangabotshwanga baye basuswa ngokuhlamba, kwaye isisombululo se-substrate ye-TMB songezwa kumaqula, kwaye imveliso eluhlaza okwesibhakabhaka yenziwa ngokusabela kwe-conjugates ye-microplate, kwaye ubunzulu bombala buhambelana ngokufanelekileyo kunye nenani elithile le-antibodies equlethwe kwisampuli.Emva kokuba isisombululo sokuphelisa songezwa ukuphelisa ukusabela, imveliso yajika yaphuzi;Ixabiso lokufunxa kwi-reaction nganye kakuhle lichongwa ngumfundi we-microplate kwi-wavelength ye-450 nm ukugqiba ukuba isampuli iqulethe ii-antibodies ze-pox yegusha.