ISISHWANKATHELO NENKCAZO UVAVANYO
Urhudo ngomnye wemigaqo engunobangela wokugula kwabantwana kunye nokufa kwihlabathi jikelele, okukhokelela ekuswelekeni kwezigidi ezi-2.5 ngonyaka.Usulelo lweRotavirus ngoyena nobangela uphambili worhudo olubi kwiintsana nakubantwana abangaphantsi kweminyaka emihlanu ubudala, lubalelwa kwi-40% -60% ye-acute gastroenteritis kwaye ibangele ukufa kwabantwana okuqikelelwa kuma-500,000 ngonyaka.Kwiminyaka emihlanu, phantse wonke umntwana emhlabeni sele esulelwe yi-rotavirus ubuncinane kanye.Ngeentsholongwane ezilandelayo, impendulo ebanzi, i-heterotypic antibody ifunwa;ngoko ke, abantu abadala abafane bachaphazeleke.
Ukuza kuthi ga ngoku amaqela asixhenxe e-rotavirus (amaqela AG) abekwe yedwa kwaye
uphawu.I-Rotavirus yeQela, eyona nto ixhaphakileyo, ibangela ngaphezu kwe-90% yazo zonke izifo ze-Rotavirus kubantu.I-Rotavirus isasazwa ikakhulu ngomzila we-fecaloral, ngokuthe ngqo ukusuka kumntu ukuya kumntu.Iintsholongwane zentsholongwane kwisitulo zifikelela ubuninzi ngokukhawuleza emva kokuqala kokugula, emva koko ziyehla.Ixesha lokufukamela usulelo lwe-rotavirus ludla ngokuba lusuku olunye ukuya kwezintathu kwaye lulandelwa sisisu esine-avareji yobude beentsuku ezintathu ukuya kwezisixhenxe.Iimpawu zesi sifo zisusela kurhudo olungephi, olunamanzi ukuya kurhudo olubi olunomkhuhlane kunye nokugabha.
Ukuxilongwa kosulelo lwe-rotavirus kunokwenziwa emva kokuxilongwa kwe-gastroenteritis njengonobangela worhudo olubi ebantwaneni.Kutshanje, ukuxilongwa okuthe ngqo kosulelo nge-rotavirus kuye kwafumaneka ngokufunyanwa kwe-antigen yentsholongwane kwisitulo ngeendlela ze-immunoassay ezifana ne-latex agglutination assay, i-EIA, kunye ne-lateral flow chromatographic immunoassay.
UVavanyo lwe-Rotavirus Ag oluKhawulezayo yi-lateral flow chromatographic immunoassay esebenzisa iperi ye-antibodies ethile ukuze ibone ngokomgangatho i-antigen ye-rotavirus kwi-fecal specimen.Uvavanyo lunokwenziwa ngaphandle kwezixhobo zebhubhoratri ezinzima, kwaye iziphumo ziyafumaneka kwimizuzu eyi-15.
UMGAQO
I-Rotavirus Ag Rapid Test yi-lateral flow chromatographic immunoassay.Umtya wovavanyo uqulathe: 1) ipad yeconjugate enemibala eburgundy ene-monoclonal anti-rotavirus antibody edityaniswe negolide ye-colloidal (i-anti-rotavirus conjugates) kunye ne-antibody yokulawula edityaniswe negolide ye-colloidal, 2) i-nitrocellulose membrane strip equlethe umgca wovavanyo (T umgca) kunye nomgca wokulawula (umgca weC).Umgca we-T ufakwe ngaphambili kunye nenye i-anti-rotavirus ye-monoclonal, kwaye umgca we-C ufakwe ngaphambili kunye ne-antibody yomgca wokulawula.
Xa umthamo owaneleyo wesampulu ekhutshiweyo ukhutshwa kwisampulu yequla lekhasethi yovavanyo, umfuziselo ufuduka ngesenzo se-capillary kwikhasethi.I-Rotavirus Ag, ukuba ikhona kwi-specimen, iya kubophelela kwi-conjugates ye-anti-rotavirus.Emva koko i-immunocomplex ibanjwe kwi-membrane yi-antibody ye-rotavirus efakwe ngaphambili eyenza umgca we-burgundy onemibala ye-T, ebonisa umphumo wokuvavanya i-rotavirus. ebonisa iziphumo ezingalunganga ze-rotavirus.Uvavanyo luqulethe ulawulo lwangaphakathi (umgca we-C), omele ubonise umgca we-burgundy onemibala ye-immunocomplex ye-antibodies yokulawula, kungakhathaliseki ukuba ukuphuhliswa kombala kumgca we-T.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
