Inkcazo eneenkcukacha
Qwalasela naziphi na izixhobo zemvelaphi yomntu njengezosulelo kwaye uziphathe usebenzisa iinkqubo eziqhelekileyo zokhuseleko lwendalo.
I-Plasma
1. Qokelela i-specimen yegazi kwi-lavender, eluhlaza okwesibhakabhaka okanye eluhlaza ityhubhu yokuqokelela phezulu (equkethe i-EDTA, i-citrate okanye i-heparin, ngokulandelanayo kwi-Vacutainer®) nge-veinpuncture.
2.Ukwahlula i-plasma nge-centrifugation.
3.Ukurhoxisa ngononophelo iplasma kwityhubhu ebhalwe kwangaphambili.
ISerum
1. Qokelela isampuli yegazi kwi-tube yokuqokelela ephezulu ebomvu (engenayo i-anticoagulants kwi-Vacutainer®) nge-veinpuncture.
2.Vumela igazi ukuba lijije.
3.Ukwahlula i-serum nge-centrifugation.
4.Ukurhoxisa ngononophelo i-serum kwi-tube entsha ebhalwe kwangaphambili.
5.Vavanya iisampuli ngokukhawuleza emva kokuqokelela.Gcina iisampuli ku-2°C ukuya ku-8°C ukuba awuvavanywanga ngoko nangoko.
6.Gcina iisampuli kwi-2°C ukuya ku-8°C ukuya kutsho kwiintsuku ezi-5.Imizekelo kufuneka ifakwe emkhenkceni kwi -20°C ukuze igcinwe ixesha elide
Igazi
Amathontsi egazi elipheleleyo anokufunyanwa ngokugqobhoza ngencam yomnwe okanye ngokugqobhoza imithambo.Musa ukusebenzisa naliphi na igazi eline-hemolized ukuvavanya.Iisampuli zegazi elipheleleyo kufuneka zigcinwe efrijini (2 ° C-8 ° C) ukuba ayivavanywanga ngokukhawuleza.Imizekelo mayivavanywe kwiiyure ezingama-24 zokuqokelelwa kwayo. Kuphephe ukukhenkciswa kwemijikelo emininzi.Ngaphambi kovavanyo, zisa iisampulu ezikhenkcezisiweyo kwiqondo lobushushu begumbi ngokucothayo kwaye udibanise ngobunono.Iisampulu eziqulethe i-particle ebonakalayo kufuneka icaciswe nge-centrifugation ngaphambi kokuvavanya.
INKQUBO YOVAVANYO
Inyathelo 1: Yiza nomzekelo kunye nezixhobo zovavanyo kwiqondo lobushushu begumbi ukuba ifriji okanye ikhenkcezisiwe.Emva kokuba inyibilikisiwe, xuba isampuli kakuhle ngaphambi kokuvavanya.
Inyathelo 2: Xa ulungele ukuvavanya, vula isingxobo kwinotshi kwaye ususe isixhobo.Beka isixhobo sovavanyo kwindawo ecocekileyo nesicaba.
Inyathelo lesi-3: Qinisekisa ukuba uleyibhelishe isixhobo ngenombolo yesazisi yomzekelo.
Inyathelo lesi-4: Kuvavanyo lwegazi elipheleleyo – Galela ithontsi legazi eli-1 (malunga ne-30-35 µL) kwisampulu yequla.– Emva koko yongeza amathontsi ama-2 (malunga ne-60-70 µL) yeSampuli yeDiluent ngoko nangoko.