Inkcazo eneenkcukacha
I-Syphilis Tp yintsholongwane ye-spirochete, eyi-pathogen ye-syphilis ye-venereal.Nangona izinga legcushuwa eUnited States liyancipha emva kokuqhambuka kwegcushuwa, izinga legcushuwa eYurophu liye lenyuka ukususela ngowe-1986 ukuya kutsho ngowe-1991.UMbutho wezeMpilo weHlabathi unike ingxelo ye-12 lezigidi zeemeko ezitsha ngo-1995. Okwangoku, izinga eliqinisekileyo lovavanyo lwe-serological lwegcushuwa kubantu abosuleleke yi-HIV liyenyuka kutshanje.
Ukufunyaniswa ngokukhawuleza kwendibaniselwano ye-antibody ye-syphilis yinkqubo yokugonywa kwe-chromatography esecaleni.
Ikhithi yovavanyo ibandakanya: 1) i-recombinant Tp antigen IgG gold conjugate edibanisa purplish red conjugate pad colloidal gold (Tp conjugate) kunye nemivundla.
2) I-Nitrocellulose membrane strip band equkethe ibhendi yovavanyo (T) kunye nebhendi yokulawula (i-C band).Ibhendi ye-T yayiqatywe ngaphambili nge-non-conjugate recombinant Tp antigen, kwaye i-C band yayiqatywe ngaphambili nge-antibody ye-IgG yebhokhwe yokulwa nomvundla.
Xa umthamo owaneleyo wesampuli usasazwa kumngxuma wesampuli, isampuli ifuduka kwibhokisi ngesenzo se-capillary kwibhokisi.Ukuba i-anti-Tp ye-antibody ikhona kwisampulu, iya kubophelela kwi-Tp conjugate.Olu khuselo lomzimba luthi ke lubanjwe kwinwebu yi-antigen eqatywe ngaphambili ye-Tp, yenze ibhendi ye-T emfusa ebomvu, ebonisa iziphumo ezilungileyo zokubhaqwa kwe-Tp antibody.Ukungabikho kwe-T band kubonisa ukuba isiphumo sibi.Uvavanyo kuquka ulawulo lwangaphakathi (band C) kufuneka ubonise emfusa ebomvu band ibhokhwe anti umvundla IgG / umvundla IgG igolide conjugate of complex omzimba, kungakhathaliseki T-band yayo.Ngaphandle koko, umphumo wovavanyo awusebenzi kwaye kufuneka kusetyenziswe esinye isixhobo.