Inkcazo eneenkcukacha
Inyathelo 1: Yiza nomzekelo kunye nezixhobo zovavanyo kwiqondo lobushushu begumbi ukuba ifriji okanye ikhenkcezisiwe.Emva kokuba inyibilikisiwe, xuba isampuli kakuhle ngaphambi kokuvavanya.
Inyathelo 2: Xa ulungele ukuvavanya, vula isingxobo kwinotshi kwaye ususe isixhobo.Beka isixhobo sovavanyo kwindawo ecocekileyo nesicaba.
Inyathelo lesi-3: Qinisekisa ukuba uleyibhelishe isixhobo ngenombolo yesazisi yomzekelo.
Inyathelo lesi-4:
Kuvavanyo lwegazi lonke
- Galela ithontsi legazi eli-1 (malunga nama-20 µL) kwisampulu yequla.
- Emva koko yongeza amathontsi ama-2 (malunga ne-60-70 µL) yeSampuli yoDiluent ngoko nangoko.
Kuvavanyo lweserum okanye lweplasma
- Gcwalisa i-pipette dropper kunye nomzekelo.
- Ukubamba idropha ngokuthe nkqo, khupha ithontsi e-1 (malunga ne-30 µL-35 µL) yomboniso kwisampulu yequla uqinisekise ukuba akukho maqamza omoya.
- Emva koko yongeza amathontsi ama-2 (malunga ne-60-70 µL) yeSampuli yoDiluent ngoko nangoko.
Inyathelo 5: Seta isibali-xesha.
Inyathelo lesi-6: Iziphumo zingafundwa kwimizuzu engama-20.Iziphumo ezincomekayo zinokubonakala ngomzuzu o-1.Musa ukufunda iziphumo emva kwemizuzu engama-30. Ukuze ugweme ukudideka, lahla isixhobo sokuvavanya emva kokutolika umphumo.