ISISHWANKATHELO NENKCAZO UVAVANYO
Umkhuhlane wetyphoid ubangelwa yi-S. typhi, intsholongwane ye-Gram-negative.Ehlabathini lonke kuqikelelwa ukuba zizigidi ezili-17 iimeko zokufa kunye nama-600,000 okufa okunxulumene noko kwenzeka ngonyaka.Izigulane ezosulelwe yi-HIV zisengozini eyongezelelekileyo yokosulelwa yikliniki yi-S. typhi.Ubungqina bosulelo lwe-H. pylori bukwabonisa ukonyuka komngcipheko wokufumana i-typhoid fever.I-1-5% yezigulane ziba ngumthwali ongapheliyo ogcina i-S. typhi kwi-gallbladder.
Ukuxilongwa kwekliniki ye-typhoid fever kuxhomekeke ekubekweni yedwa kwe-S. typhi egazini, umongo okanye isilonda esithile se-anatomic.Kwizibonelelo ezingenakukwazi ukwenza le nkqubo enzima kunye nexesha elide, uvavanyo lweFilix-Widal lusetyenziselwa ukuququzelela ukuxilongwa.Nangona kunjalo, imida emininzi ikhokelela kubunzima ekutolikeni kovavanyo lweWidal.
Ngokwahlukileyo, iTyphoid IgG/IgM Rapid Test Kit luvavanyo olulula nolukhawulezayo lwaselabhoratri.Uvavanyo ngaxeshanye lubona kwaye lwahlula-hlula i-IgG kunye ne-IgM antibodies kwi-S. typhi i-antigen ethile kwi-specimen yegazi lonke ngaloo ndlela inceda ekumiseleni ukubonakaliswa kwangoku okanye kwangaphambili kwi-S. typhi.
UMGAQO
ITyphoid IgG/IgM Combo Rapid Test is a lateral flow chromatographic
uvavanyo lwe-immunoassay.I-cassette yokuvavanya iquka: 1) i-burgundy colored conjugate pad equkethe i-recombinant S. typhoid H antigen kunye ne-O antigen ehlanganiswe negolide ye-colloid (i-Typhoid conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocellulose i-membrane (i-membrane ye-membrane equkethe iibhendi ezimbini zovavanyo (M kunye ne-G band).I-M band ifakwe ngaphambili kunye ne-monoclonal anti-human IgM yokuchongwa kwe-IgM anti-S.typhi, i-G band iqatywe ngaphambili ngeerejenti zokubhaqa i-IgG
anti-S.typhi, kunye neqela le-C liqatywe ngaphambili kunye nebhokhwe yokulwa nomvundla IgG.
Xa umthamo owaneleyo wovavanyo lovavanyo lukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.Anti-S.I-typhi IgM ukuba ikhona kumzekelo iya kubophelela kwii-conjugates zeTyphoid.I-immunocomplex iye ibanjwe kwi-membrane yi-anti-human IgM antibody, eyenza i-burgundy enemibala ye-M band, ebonisa umphumo we-S. typhi IgM we-positive test.
Anti-S.I-typhi IgG ukuba ikhona kumzekelo iya kubophelela kwii-conjugates zeTyphoid.I-immunocomplex ke ibanjwe yi-reagents efakwe ngaphambili kwi-membrane, yenze i-burgundy enemibala ye-G band, ebonisa isiphumo sovavanyo lwe-S. typhi IgG.
Ukungabikho kwawo nawaphi na amabhanti ovavanyo (M no-G) acebisa isiphumo esibi.Uvavanyo iqulathe ulawulo lwangaphakathi (C band) ekufuneka abonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG/umvundla IgG-igolide conjugate kungakhathaliseki ukuba uphuhliso umbala kuyo nayiphi na bands uvavanyo.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
