Zika IgG/IgM Rapid Test Kit (Colloidal Gold)

INGCACISO:Iimvavanyo ezingama-25/ikhithi

UKUSETYENZISWA OKUHLOSIWEYO:Uvavanyo oluKhawulezayo lweZika IgM/IgG luvavanyo lwe-chromatographic immunoassay olusecaleni lokuqukuqela komgangatho we-IgM/IgG ye-anti-zika virus (ZIKA) kwi-serum yomntu, iplasma okanye igazi elipheleleyo.Yenzelwe ukuba isetyenziswe njengovavanyo lokuhlola kwaye njengoncedo ekuxilongeni usulelo nge-ZIKA.Nawuphi na umzekelo osebenzayo kunye noVavanyo oluKhawulezayo lweZika IgM/IgG kufuneka luqinisekiswe ngenye indlela (ii) zovavanyo kunye neziphumo zeklinikhi.


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ISISHWANKATHELO NENKCAZO UVAVANYO

Intsholongwane kaZika (Zika): isasazeka ngokulunywa yingcongconi i-Aedes, umama nomntwana, utofelo-gazi kunye nosulelo lwesondo.I-antibody ye-IgG/IgM iveliswa kwiveki enye emva kokuba iqalile, ngoko ke ukufunyanwa kwe-IgG/IgM kubaluleke kakhulu kwangethuba.

ukuxilongwa kwentsholongwane yeZika.I-Zika ifunyanwe ngokusekelwe kuhlalutyo lwe-serological kunye nokuhlukaniswa kwentsholongwane kwiigundane okanye kwinkcubeko ye-tissue.I-IgM immunoassay yeyona ndlela isebenzayo yokuvavanya elebhu.I-Zika IgM/IgG Rapid Test isebenzisa i-antigens ephinda iphinda ithathwe kwiprotheni yesakhiwo sayo, ibona i-IgM/IgG i-anti-zika kwi-serum yesigulana okanye kwi-plasma kwimizuzu eyi-15.Uvavanyo lunokwenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwesixhobo esinzima selabhoratri.

UMGAQO

Uvavanyo lweZika IgM/IgG oluKhawulezayo luvavanyo lwe-chromatographic immunoassay olusecaleni.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equkethe i-antigen edibeneyo ehlanganiswe negolide ye-colloid (i-Zika conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocellulose membrane strip equlethe iibhendi ezimbini zovavanyo (iibhendi ze-M kunye ne-G) kunye nolawulo. ibhendi (C band).I-M band ifakwe ngaphambili kunye ne-monoclonal anti-human IgM yokuchongwa kwe-IgM ye-anti-Zika, i-G band ifakwe ngaphambili kunye ne-reagents yokuchongwa kwe-IgG ye-anti-Zika, kwaye i-C band ifakwe ngaphambili nge-anti yebhokhwe. umvundla IgG.

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Xa umthamo owaneleyo wovavanyo lovavanyo lukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.I-Anti-Zika IgM ukuba ikhona kwi-specimen iya kubophelela kwi-conjugates yeZika.I-immunocomplex ke ibanjwe kwi-membrane yi-anti-human IgM antibody, eyenza i-burgundy enemibala ye-M band, ebonisa umphumo we-Zika IgM.

I-Anti-Zika IgG ukuba ikhona kwi-specimen iya kubophelela kwi-conjugates yeZika.I-immunocomplex ke ibanjwe yi-reagents efakwe ngaphambili kwi-membrane, yenza i-burgundy enemibala ye-G band, ebonisa umphumo we-Zika IgG.Ukungabikho kwawo nawaphi na amabhanti ovavanyo (M no-G) ​​acebisa isiphumo esibi.Uvavanyo iqulathe ulawulo lwangaphakathi (C band) ekufuneka abonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG / umvundla IgG-igolide conjugate kungakhathaliseki ukuba uphuhliso umbala kuyo nayiphi na bands uvavanyo.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.


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