Chikungunya IgG/IgM+NS1 Antigen Rapid Test Kit (Colloidal Gold)

I-Chikungunya lusulelo olunqabileyo lwentsholongwane olusasazwa ngokulunywa yingcongconi i-Aedes aegypti eyosulelekileyo.Ibonakala ngerhashalala, umkhuhlane, kunye neentlungu ezibuhlungu zamalungu (arthralgias) ezidla ngokuthatha iintsuku ezintathu ukuya kwezisixhenxe.Eli gama lithatyathwe kwigama lesiMakonde elithetha “into egobileyo” xa kubhekiswa kwindlela yokuma egobileyo eyenziwa ngenxa yeempawu zesi sifo.Kwenzeka ngexesha leemvula

kwiindawo ezishushu zehlabathi, ikakhulu eAfrika, eMzantsi-Mpuma Asia, kumazantsi eIndiya nasePakistan.Iimpawu zihlala zingacaciswanga ngokwezonyango ezo zijongwe kumkhuhlane wedengue.Ngokwenene, usulelo oluphindwe kabini lwedengue kunye ne-chikungunya kuye kwaxelwa eIndiya.Ngokungafaniyo nedengue, iimpawu zokopha azifane zifane kwaye amaxesha amaninzi esi sifo sisigulo esizithintelayo.Ngoko ke kubaluleke kakhulu

ukwahlula ngokwezonyango idengue kusulelo lwe-CHIK.I-CHIK ifunyanwe ngokusekelwe kuhlalutyo lwe-serological kunye nokuhlukaniswa kwentsholongwane kwiigundane okanye kwinkcubeko ye-tissue.I-IgM immunoassay yeyona ndlela isebenzayo yokuvavanya elebhu.I-Chikungunya IgG / IgM Rapid Test isebenzisa i-antigens edibeneyo ephuma kwiprotheni yesakhiwo sayo, ifumanisa i-IgG / IgM i-anti-CHIK kwi-serum yesigulane okanye kwi-plasma kwimizuzu engama-20.Uvavanyo lunokwenziwa ngabangaqeqeshwanga okanye

abasebenzi abanezakhono ezingephi, ngaphandle kwezixhobo zaselabhoratri ezinzima.

IChikungunya IgG/IgM+NS1 antigen Rapid Test is a lateral flow chromatographic immunoassay.Ikhasethi yovavanyo ine-IgG/IgM Strip kunye ne-NS1 strip.

IgG/IgM strip:1) i-burgundy colored conjugate pad equlathe Chikungunya recombinant envelope antigens ezidityaniswe ne colloid gold (idengue conjugates) kunye nomvundla IgG-gold conjugates,2) i nitrocellulose membrane strip equlathe bands ezimbini zovavanyo (G and M bands) kunye ne ibhendi yolawulo (C band).I-G band ifakwe ngaphambili kunye ne-antibody ukuze kubonwe intsholongwane ye-IgG ye-anti-Chikungunya, i-M band ifakwe i-antibody ukuze ibonwe i-virus ye-IgM ye-anti-Chikungunya, kwaye i-C band ifakwe ngaphambili ngebhokhwe yokulwa nomvundla i-IgG.

NS1 strip: 1) i-burgundy colored conjugate pad equkethe i-mouse anti-Chikungunya NS1 i-antigen ehlanganiswe negolide ye-colloid (i-Chikungunya Ab conjugates), i-2) i-membrane ye-nitrocellulose equlethe ibhendi yovavanyo (i-T band) kunye nebhendi yokulawula (i-C band).I-T band ifakwe ngaphambili kunye nomvundla we-anti-Chikungunya NS1 antigen, kwaye i-C band ifakwe ngaphambili nge-anti-mouse ye-IgG ye-antibody.

I-IgG/IgM strip:Xa umthamo owaneleyo wovavanyo wovavanyo ukhutshelwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka nge-capillary action kwikhasethi.Intsholongwane ye-IgG ye-anti-Chikungunya ukuba ikhona kumzekelo iya kubophelela kwi-Chikungunya conjugates.I-immunocomplex ke ibanjwe yi-reagent efakwe kwi-G band, yenza i-burgundy enemibala ye-G band, ebonisa i-Chikungunya virus ye-IgG isiphumo sovavanyo oluhle kwaye iphakamisa usulelo lwamva nje okanye oluphindayo.I-virus ye-IgM ye-anti-Chikungunya, ukuba ikhona kwi-specimen, iya kubophelela kwi-conjugates ye-Chikungunya.I-immunocomplex iye ibanjwe yi-reagent efakwe ngaphambili kwi-M band, yenza i-burgundy enemibala ye-M band, ebonisa i-Chikungunya virus ye-IgM isiphumo sovavanyo olulungileyo kwaye iphakamisa usulelo olutsha.Ukungabikho naziphi na iibhendi zovavanyo (G kunye M) icebisa isiphumo esibi.Uvavanyo luqulathe ulawulo lwangaphakathi (C band) ekufuneka ibonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG/

umvundla we-IgG-gold conjugate kungakhathaliseki uphuhliso lombala nakweyiphi na i-T bands.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.

NS1 Strip:Xa umthamo owaneleyo wovavanyo ukhutshelwa kwisampulu yequla lekhasethi, isampuli iyafuduka ngecapillary action kwikhasethi yovavanyo.Chikungunya NS1 Ag ukuba ikhona kumzekelo iyakubophelela kwi Chikungunya Ab conjugates.I-immunocomplex ke ibanjwe kwi-membrane yi-antibody yomvundla e-anti-NS1, eyenza i-T band enemibala e-burgundy, ebonisa isiphumo sovavanyo lwe-Chikungunya Ag.Ukungabikho kwe-T band kubonisa umphumo ongalunganga.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise ibhendi enemibala e-burgundy ye-immunocomplex yebhokhwe echasene ne-mouse IgG / i-mouse IgG-gold conjugate kungakhathaliseki ukuba kukho i-T band enemibala.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.