ISISHWANKATHELO NENKCAZO UVAVANYO
Ikholera sisifo esosulelayo esibonakala ngokuphulukana nolwelo olukhulu lomzimba kunye ne-electrolyte ngenxa yorhudo oluqatha.I-etiological agent ye-cholera ichongiwe njenge-Vibrio cholerea (V. Cholerae), ibhaktheriya yegram-negative, esasazeka ebantwini ngamanzi kunye nokutya okungcolileyo.
Uhlobo lwe-V. Cholerae luhlulwe lube ngama-serogroups amaninzi ngesiseko se-O antigens.Amacandelwana i-O1 kunye ne-O139 anomdla okhethekileyo kuba omabini anokubangela ubhubhane kunye nobhubhane wekholera.Kubalulekile ukufumanisa ngokukhawuleza ubukho be-V. cholerae O1 kunye ne-O139 kwiisampuli zeklinikhi, amanzi, kunye nokutya ukwenzela ukuba ukubeka iliso okufanelekileyo kunye namanyathelo okuthintela asebenzayo anokuthi athathwe ngabasemagunyeni bezempilo yoluntu.
Uvavanyo olukhawulezileyo lwe-Cholera Ag lunokusetyenziswa ngokuthe ngqo kwintsimi ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci kwaye umphumo ufumaneka ngaphantsi kwe-10 min, ngaphandle kwezixhobo zebhubhoratri ezinzima.
UMGAQO
Uvavanyo lweKholera Ag Rapid yi-lateral flow chromatographic immunoassay.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equkethe i-monoclonal anti-V.I-Cholera O1 kunye ne-O139 antibodies ehlanganiswe negolide ye-colloid (i-O1 / O139-antibody conjugates) kunye nomvundla i-conjugates ye-IgG yegolide, i-2) i-nitrocellulose inwebu strip equlethe ibhendi yovavanyo emibini (i-1 kunye ne-139 band) kunye nebhendi yokulawula (i-C band).Ibhendi ye-1 ifakwe ngaphambili kunye ne-monoclonal anti-V.I-antibody yeCholera O1.Ibhendi ye-139 ifakwe ngaphambili kunye ne-monoclonal anti-V.I-antibody yeCholera O139.Ibhendi ye-C iqatywe ngaphambili nge-anti-mouse ye-IgG yebhokhwe.
Xa umthamo owaneleyo wovavanyo lovavanyo lusetyenziswa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.V. I-antigen ye-Cholera i-O1 / O139 ukuba ikhona kwi-specimen iya kubophelela kwi-conjugate yegolide ye-O1 / O139-antibody ehambelanayo.Le immunocomplex ke ibanjwe kwi inwebu yi anti-V pre-coated.I-antibody ye-Cholera i-O1 / O139, eyenza ibhendi yovavanyo olunemibala e-burgundy, ebonisa umphumo we-Cholera we-Cholera O1 / O139.Ukungabikho kwebhendi yokuvavanya kubonisa umphumo ongalunganga.
Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) olufanele lubonise ibhendi enemibala e-burgundy ye-immunocomplex yebhokhwe echasene ne-mouse IgG / i-mouse IgG-gold conjugate kungakhathaliseki ukuba uphuhliso lombala kwi-test band.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
