ISISHWANKATHELO NENKCAZO UVAVANYO
Iintsholongwane zeDengue, usapho lwee-serotypes ezine ezihlukeneyo zentsholongwane (i-Den 1,2,3,4), i-singlestrained, i-enveloped, i-virus ye-RNA enengqiqo.Ezi ntsholongwane zisasazwa ziingcongconi zosapho lwakwaStegemyia oluluma emini, ingakumbi iAedes aegypti, kunye neAedes albopictus.Namhlanje, abantu abangaphezu kwe-2,5 bhiliyoni abahlala kwimimandla ekweleenjiko yaseAsia, eAfrika, eOstreliya nakumazwe aseMerika basesichengeni sokwasulelwa yidengue.Kuqikelelwa ukuba i-100 lezigidi zeemeko zomkhuhlane wedengue kunye neemeko ezingama-250,000 zedengue ebeka ubomi esichengeni umkhuhlane owophayo zenzeka minyaka le kwihlabathi liphela1-3.
Ukufunyanwa kwe-serological ye-antibody ye-IgM yeyona ndlela ixhaphakileyo yokuxilongwa kosulelo lwentsholongwane yedengue.Kutshanje, ukufunyanwa kwee-antigens ezikhutshwe ngexesha lokuphindaphinda kwentsholongwane kwisigulana esosulelekileyo kubonise iziphumo ezithembisayo.Ivumela ukuxilongwa ukususela ngosuku lokuqala emva kokuqala komkhuhlane ukuya kumhla we-9, emva kokuba isigaba seklinikhi sesi sifo siphelile, ngaloo ndlela ivumela unyango lwangethuba lubekwe ngokukhawuleza4-.Uvavanyo olukhawulezayo lweDengue IgG/IgM luphuhliswa ukufumanisa ukujikeleza kwe-antigen yedengue kwi-serum, iplasma okanye igazi elipheleleyo.Uvavanyo lunokwenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwezixhobo zaselabhoratri.
UMGAQO
Uvavanyo lweDengue IgG/IgM oluKhawulezayo luvavanyo lwe-chromatographic immunoassay olusecaleni.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equlethe i-dengue recombinant envelope antigens ehlanganiswe negolide ye-colloid (i-dengue conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocellulose inwebu equlethe iibhendi ezimbini zovavanyo (i-G kunye ne-M band band) kunye nolawulo lwe-band).I-G band ifakwe ngaphambili kunye ne-antibody ukuze kubonwe intsholongwane ye-IgG yokulwa ne-dengue, i-M band iqatywe nge-antibody ukuze ibonwe i-IgM yokulwa ne-virus ye-dengue, kwaye i-C band ifakwe ngaphambili nge-igG yebhokhwe yokulwa nomvundla.
Xa umthamo owaneleyo wovavanyo lovavanyo lukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.Intsholongwane ye-IgG echasene nedengue ukuba ikhona kumzekelo iya kubophelela kwiiconjugates zedengue.I-immunocomplex iye ibanjwe yi-reagent egqunywe kwi-G band, yenza i-burgundy enemibala ye-G band, ebonisa i-virus ye-dengue IgG isiphumo sovavanyo olulungileyo kwaye iphakamisa usulelo lwamva nje okanye oluphindayo.Intsholongwane ye-IgM echasene nedengue, ukuba ikhona kumzekelo, iya kubophelela kwi-conjugates yedengue.I-immunocomplex iye ibanjwe yi-reagent efakwe ngaphambili kwi-M band, eyenza i-burgundy enemibala ye-M band, ebonisa i-virus ye-dengue IgM isiphumo sovavanyo olulungileyo kwaye iphakamisa usulelo olutsha.
Ukungabikho naziphi na iibhendi zovavanyo (G kunye no-M) icebisa isiphumo esibi. Uvavanyo luqulathe ulawulo lwangaphakathi (C band) ekufuneka ibonise band burgundy enombala we immunocomplex yebhokhwe anti umvundla IgG/umvundla IgG-gold conjugate kungakhathaliseki ukuba uphuhliso umbala kuyo nayiphi na bands T.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
