ISISHWANKATHELO NENKCAZO UVAVANYO
Iintsholongwane zeDengue, usapho lwee-serotypes ezine ezihlukeneyo zentsholongwane (i-Den 1,2,3,4), i-singlestrained, i-enveloped, i-virus ye-RNA enengqiqo.Ezi ntsholongwane zisasazwa ziingcongconi zosapho lwakwaStegemyia oluluma emini, ingakumbi iAedes aegypti, kunye neAedes albopictus.Namhlanje, abantu abangaphezu kwe-2,5 bhiliyoni abahlala kwimimandla ekweleenjiko yaseAsia, eAfrika, eOstreliya nakumazwe aseMerika basesichengeni sokwasulelwa yidengue.Kuqikelelwa ukuba i-100 lezigidi zeemeko zomkhuhlane wedengue kunye neemeko ezingama-250,000 zedengue esophayo esisongela ubomi zenzeka minyaka le kwihlabathi liphela.
Ukufunyanwa kwe-serological ye-antibody ye-IgM yeyona ndlela ixhaphakileyo yokuxilongwa kosulelo lwentsholongwane yedengue.Kutshanje, ukufunyanwa kwee-antigens ezikhutshwe ngexesha lokuphindaphinda kwentsholongwane kwisigulana esosulelekileyo kubonise iziphumo ezithembisayo.Ivumela ukuxilongwa ukususela ngosuku lokuqala emva kokuqala komkhuhlane ukuya kumhla we-9, emva kokuba isigaba sekliniki sesi sifo siphelile, ngaloo ndlela ivumela unyango lwakwangoko lubekwe ngokukhawuleza4-.Uvavanyo olukhawulezayo lweDengue NS1 luphuhliswa ukufumanisa ukujikeleza kwe-antigen yedengue kwiserum. ,iplasma okanye igazi elipheleleyo.Uvavanyo lunokwenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwezixhobo zaselabhoratri.
UMGAQO
Uvavanyo lweDengue NS1 oluKhawulezayo luvavanyo lwe-chromatographic flow lateral.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equlethe i-mouse anti-dengue NS1 i-antigen ehlanganiswe negolide ye-colloid (i-Dengue Ab conjugates), i-2) i-nitrocellulose membrane strip equlethe ibhendi yovavanyo (i-T band) kunye nebhendi yokulawula (C iqela).Ibhendi ye-T ifakwe ngaphambili kunye ne-antigen ye-mouse ye-dengue NS1, kunye ne-C band
iqatywe ngaphambili nge-anti-mouse ye-IgG yebhokhwe.Izilwa-buhlungu kwi-antigen yedengue ziqaphela ii-antigens kuzo zonke ezine serotypes zentsholongwane yedengue.
Xa umthamo owaneleyo wesampulu yovavanyo ukhutshwa kwisampulu yequla lekhasethi, umzekelo ufuduka ngesenzo se-capillary kwikhasethi yovavanyo.I-Dengue NS1 Ag ukuba ikhona kumzekelo iyakubophelela kwi-Dengue Ab conjugates.I-immunocomplex iye ibanjwe kwi-membrane yi-anti-coated mouse antiNS1 antibody, eyenza i-T band enemibala e-burgundy, ebonisa iziphumo zovavanyo lwe-Dengue Ag.
Ukungabikho kwe-T band kubonisa umphumo ongalunganga.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise ibhendi enemibala e-burgundy ye-immunocomplex yebhokhwe echasene ne-mouse IgG / i-mouse IgG-gold conjugate kungakhathaliseki ukuba kukho i-T band enemibala.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
