Filariasis Antibody Rapid Test-kit ( Colloidal Gold )

INGCACISO:Iimvavanyo ezingama-25/ikhithi

UKUSETYENZISWA OKUHLOSIWEYO:I-Filariasis Ab Rapid Test is a lateral flow immunoassay for the qualitative discovery of antibodies eziquka IgG, IgM, kunye ne-IgA ukuya kwii-subspecies ze-anti-lymphatic filarial parasites (W. Bancrofti kunye ne-B. Malayi) kwi-serum yabantu, i-plasma okanye igazi elipheleleyo .Olu vavanyo lujoliswe ukuba lusetyenziswe njengovavanyo lokuhlola kunye noncedo ekuxilongweni kwesifo seFilariasis.Nawuphi na umzekelo osebenzayo kunye noVavanyo lwe-Filariasis Ab Rapid kufuneka luqinisekiswe ngenye indlela yovavanyo.


Iinkcukacha zeMveliso

Iithegi zeMveliso

ISISHWANKATHELO NENKCAZO UVAVANYO

I-lymphatic filariasis eyaziwa ngokuba yi-Elephantiasis, ngokuyintloko ebangelwa ngu-W. bancrofti no-B. malayi, ichaphazela abantu abamalunga ne-120 lezigidi kumazwe angaphezu kwama-80.Esi sifo sidluliselwa ebantwini ngokulunywa ziingcongconi ezosulelekileyo apho imicroflariae efunxayo kumntu owosulelekileyo ikhula ibe yimibungu yenqanaba lesithathu.Ngokuqhelekileyo, ukubonakaliswa okuphindaphindiweyo kunye nexesha elide kwimibungu esulelekileyo kuyafuneka ukuze kusekwe usulelo lomntu.

Uxilongo oluchanekileyo lweparasitologic lubonakaliso lwemicroflariae kwiisampulu zegazi.Nangona kunjalo, olu vavanyo lomgangatho wegolide luthintelwe yimfuno yokuqokelela igazi ebusuku kunye nokungabikho kovakalelo olwaneleyo.Ukufunyanwa kwee-antigens ezijikelezayo kufumaneka ngokurhweba.Ukusetyenziswa kwayo kulinganiselwe kwi-W. bancrofti.Ukongeza, i-microfilaremia kunye ne-antigenemia zikhula ukusuka kwiinyanga ukuya kwiminyaka emva kokuvezwa.

Ukufunyanwa kwe-antibody kunika iindlela zakwangoko zokubona usulelo lwe-filarial parasite.Ubukho be-IgM kwi-antigens ye-parasite bucebisa usulelo lwangoku, ngelixa, i-IgG ihambelana nenqanaba losulelo lwasemva kwexesha okanye usulelo oludlulileyo.Ngaphaya koko, ukuchongwa kwee-antigens ezigciniweyo kuvumela uvavanyo lwe-'pan-filaria' ukuba lusebenze.Ukusetyenziswa kweeprotheyini ezidibeneyo kuphelisa ukuhlangana kwabantu abanezinye izifo ze-parasitic.

I-Filariasis Ab Rapid Test isebenzisa ii-antigens ze-recombinant ezigciniweyo ukuze ngaxeshanye zibone i-Antibody kwi-W. bancrofti kunye ne-B. malayi parasites ngaphandle komda ekuqokeleleni i-specimen.

UMGAQO

I-Filariasis Ab Rapid Test yi-lateral flow chromatographic immunoassay.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equlethe i-recombinant Filariasis ethile i-antigen edityaniswe negolide ye-colloid (i-Filariasis conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocellulose membrane strip equlethe ibhendi yovavanyo (ibhendi ye-T) kunye nolawulo. ibhendi (C band).I-T band ifakwe ngaphambili kunye ne-un-conjugated Filariasis antigen, kwaye i-C band ifakwe ngaphambili nge-anti-rabbit ye-IgG yebhokhwe.

r; lungisa

Xa umthamo owaneleyo wovavanyo lovavanyo lukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.I-AntiFilariasis Ab ukuba ikhona kwi-specimen iya kubophelela kwi-Filariasis conjugates.I-immunocomplex ke ibanjwe kwi-membrane yi-antigen efakwe ngaphambili, yenza i-burgundy band T enemibala, ebonisa i-aFilariasis Ab isiphumo sovavanyo oluhle.Ukungabikho kwe-T band kubonisa umphumo ongalunganga.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise i-burgundy band enemibala ye-immunocomplex yebhokhwe echasene nomvundla IgG / umvundla we-IgG-gold conjugate kungakhathaliseki ukuba uphuhliso lombala kwi-T band.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.


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