I-RV IgG yoVavanyo oluKhawulezayo Iphepha elinganqunyulwanga

I-RV IgG yoVavanyo oluKhawulezayo lwePhepha elingasikwanga Umfanekiso okhoyo

Uvavanyo oluKhawulezayo lweRV IgG

Uhlobo: Iphepha elingasikwanga

Uphawu: Bio-mapper

Ikhathalogu:RT0521

Umzekelo:WB/S/P

Uvakalelo:91.10%

Ukukhankanya:99%

I-Rubella iyenzeka kakhulu kubantwana abakwiminyaka yesikolo kunye nabafikisayo, kwaye ngaphezulu kwe-80% yabemi banethemba le-antibody yentsholongwane.Abasetyhini abakhulelweyo bosulelwa yi-rubella virus ngaphambi kweeveki ezingama-20 zokukhulelwa, kwaye izehlo ze-teratogenesis yomntwana zaziphezulu.Abantu abadala kunye nabantwana abosulelwe yi-rubella virus banokubangela ukuqhama kwesikhumba.I-ELISA: Umthwali ogqunywe nge-antigen ye-rubella virus engasebenziyo unokubopha kunye ne-antibody ethile kwisampulu evavanyiweyo, kwaye i-antibody ehambelana nayo ifunyenwe nge-enzyme ebhalwe i-anti human immunoglobulin kunye ne-substrate.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Inkcazo eneenkcukacha

(1) Ukuqokelela isampuli kunye novavanyo lokuhlola, kufuneka kuqokelelwe isampuli yegazi enye kuphela.Nangona kunjalo, ukuba kuyimfuneko ukugweba isimo somzimba sokuzivikela kubantu abosulelwe yintsholongwane, kuyimfuneko ukuthatha iisampulu kwizigulane ezikrokrelwayo ze-rubella phakathi kweentsuku ezi-3 emva kokuqala kwe-rash kunye neentsuku ezi-14 ukuya kwezi-21 zokubhaqa ngaxeshanye.
(2) Ngokufanayo ne-ELISA ngokubanzi, yongeza i-PBS 50 kumngxuma ngamnye wolawulo kunye nesampuli μ l.Qhubeka ukongeza isampuli 10 μ l.Ukushisa kwi-25 ℃ ye-45 min, hlamba kwaye uyomise.
(3) Yongeza iimpawu ze-enzyme kwiqula ngalinye 250 μ l.Indlela efanayo isetyenziselwa ukugcinwa kobushushu kunye nokuhlamba.
(4) Yongeza isisombululo se-pNPP substrate 250 μ l.Emva kokugcinwa kobushushu kunye nokuhlamba ngendlela efanayo, yongeza i-1mol / L i-sodium hydroxide 50 μ L Yeka ukusabela, ukulinganisa ixabiso le-absorbction yomgodi ngamnye kwi-405nm, kwaye ugwebe umphumo wesampuli evavanyiweyo.
(5) Ukuba sisiphumo esihle, isampuli inokuphinda ihlanjululwe ukucacisa i-antibody titer, thelekisa iziphumo zeesampuli ezimbini ezilandelelanayo, kunye nejaji.